The chitosan-based bioactive composite coating on titanium

This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin titanium substrate. Bioactive properties were investigated in vitro by immersing simulated body fluid (SBF) at 37 °C. The newly formed biomimetic layer top deposited chitosan-based Ti was confirmed X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy electrochemical measurements, while coatings' bioactivity proved alkaline phosphatase activity assay MRC-5 L929 tested cell lines. biocompatibility towards lines dye exclusion test (DET) implying non-cytotoxic effect coatings. Gentamicin release studies monitored during 21-day high-performance liquid chromatography coupled mass spectrometry, indicating rapid (≈40%) first 48 h more than 60% after 14 days.